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Sample GSM1623014 Query DataSets for GSM1623014
Status Public on Nov 18, 2015
Title Cerebellum wildtype, A888_26_C25
Sample type RNA
 
Source name Cerebellum, wildtype
Organism Mus musculus
Characteristics strain/background: C57BL/6N
genotype/variation: wildtype
developmental stage: adult
gender: male
tissue: cerebellum
Extracted molecule total RNA
Extraction protocol The manufacturer’s protocol for RNA isolation was performed. Upon RNA isolation, RNA clean-up was performed using the RNeasy Mini Kit (Qiagen, Hilden, Germany), including the DNase treatment step, according to the manufacturer’s protocol.
Label Biotin
Label protocol For each sample, 100 ng of total RNA was processed according to the Affymetrix GeneChip 3' IVT Express Kit manual (P/N 702646 Revision 6) and hybridized to Affymetrix Mouse Gene 2.1 ST arrays.
 
Hybridization protocol Affymetrix Mouse Gene 2.1 ST arrays were hybridized, washed and stained according to the protocol described in the Affymetrix GeneChip 3' IVT Express Kit Manual.
Scan protocol The arrays were scanned using the Affymetrix GeneTitan Instrument, and raw data were extracted from the scanned images and analyzed with the Affymetrix Power Tools software package.
Data processing The Affymetrix GeneTitan Instrument was used for automated array processing. Raw data was generated and extracted from the scanned images for analysis with the Affymetrix GeneChip Command Console (AGCC) Software and Expression Console Software packages.
 
Submission date Mar 03, 2015
Last update date Nov 18, 2015
Contact name Yann HERAULT
E-mail(s) herault@igbmc.fr
Organization name IGBMC/PHENOMIN-ICS
Street address 1 rue Laurent Fries
City ILLKIRCH
ZIP/Postal code 67404
Country France
 
Platform ID GPL17400
Series (1)
GSE66468 Opposite phenotypic effects and genetic dosage in mouse models of 16p11.2 deletion and duplication syndromes

Data table header descriptions
ID_REF
VALUE RMA log2 signal intensity

Data table
ID_REF VALUE
17210850 2.068502
17210852 1.621463
17210855 8.597053
17210869 8.260787
17210883 3.271241
17210887 8.090128
17210904 3.458781
17210912 8.968061
17210947 2.402737
17210953 9.498769
17210984 9.255544
17210994 2.751858
17210996 1.889053
17210998 2.480300
17211000 5.496823
17211004 7.087275
17211023 5.358987
17211033 2.665604
17211043 6.951001
17211066 4.287061

Total number of rows: 41155

Table truncated, full table size 724 Kbytes.




Supplementary file Size Download File type/resource
GSM1623014_A888_26_C25_del_wt.CEL.gz 5.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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