|
| Status |
Public on Nov 18, 2015 |
| Title |
Cerebellum wildtype, A888_28_C20 |
| Sample type |
RNA |
| |
|
| Source name |
Cerebellum, wildtype
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6N
genotype/variation: wildtype
developmental stage: adult
gender: male
tissue: cerebellum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The manufacturer’s protocol for RNA isolation was performed. Upon RNA isolation, RNA clean-up was performed using the RNeasy Mini Kit (Qiagen, Hilden, Germany), including the DNase treatment step, according to the manufacturer’s protocol.
|
| Label |
Biotin
|
| Label protocol |
For each sample, 100 ng of total RNA was processed according to the Affymetrix GeneChip 3' IVT Express Kit manual (P/N 702646 Revision 6) and hybridized to Affymetrix Mouse Gene 2.1 ST arrays.
|
| |
|
| Hybridization protocol |
Affymetrix Mouse Gene 2.1 ST arrays were hybridized, washed and stained according to the protocol described in the Affymetrix GeneChip 3' IVT Express Kit Manual.
|
| Scan protocol |
The arrays were scanned using the Affymetrix GeneTitan Instrument, and raw data were extracted from the scanned images and analyzed with the Affymetrix Power Tools software package.
|
| Data processing |
The Affymetrix GeneTitan Instrument was used for automated array processing. Raw data was generated and extracted from the scanned images for analysis with the Affymetrix GeneChip Command Console (AGCC) Software and Expression Console Software packages.
|
| |
|
| Submission date |
Mar 03, 2015 |
| Last update date |
Nov 18, 2015 |
| Contact name |
Yann HERAULT |
| E-mail(s) |
herault@igbmc.fr
|
| Organization name |
IGBMC/PHENOMIN-ICS
|
| Street address |
1 rue Laurent Fries
|
| City |
ILLKIRCH |
| ZIP/Postal code |
67404 |
| Country |
France |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE66468 |
Opposite phenotypic effects and genetic dosage in mouse models of 16p11.2 deletion and duplication syndromes |
|
