|
| Status |
Public on Sep 28, 2015 |
| Title |
AGO |
| Sample type |
RNA |
| |
|
| Source name |
Original Agilent Human Universal Reference RNA
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: pure reference RNA (AGO)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples used in the study are commercially available RNAs derived from mixture of multiple cell lines and RNAs extracted from patient bone marrow specimens
|
| Label |
Cy5
|
| Label protocol |
200 ng of starting RNAs were amplified with MessageAmp™ II aRNA Amplification Kit, 3,000 ng of amplified aRNA were labeled with Kreatech ULS™ Fluorescent Labeling Kit (with Cy5).
|
| |
|
| Hybridization protocol |
2,000 ng of cy5-labeled aRNA were hybridized following Agilent standard hybridization protocol.
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner using one color scan setting for 4x44k array slides.
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 11.5 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
Data values in Matrix sheet are Feature Extraction generated rProcessedSignal in linear scale without normalization (control probes are removed from the matrix table, so the total data lines are 43376).
|
| |
|
| Submission date |
Mar 06, 2015 |
| Last update date |
Sep 28, 2015 |
| Contact name |
Jinsheng Yu |
| E-mail(s) |
jyu@wustl.edu
|
| Organization name |
Washington University School of Medicine
|
| Department |
Genetics
|
| Lab |
GTAC Lab
|
| Street address |
660 S. Euclid Ave.
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (2) |
| GSE66626 |
Multi-platform assessment of transcriptional profiling technologies utilizing a precise probe mapping methodology (Agilent_4x44K) |
| GSE66649 |
Multi-platform assessment of transcriptional profiling technologies utilizing a precise probe mapping methodology |
|
