|
| Status |
Public on Sep 28, 2015 |
| Title |
AG1BM16_1 (gene) |
| Sample type |
RNA |
| |
|
| Source name |
Mixed RNA of both (1:16)
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: 1:16 mixture of AGO and BMO
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples used in the study are commercially available RNAs derived from mixture of multiple cell lines and RNAs extracted from patient bone marrow specimens
|
| Label |
biotin
|
| Label protocol |
50 ng of starting RNAs were amplified with NuGEN Ovation PicoSL WTA V2 kit and 2,500 ng of biotin-labeled cDNAs were hybridized.
|
| |
|
| Hybridization protocol |
Labeled cDNAs were hybridized onto Affymetrix Human Gene 1.0 ST Arrays (Gene1.0) following Affymetrix standard protocols.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix).
|
| Description |
replicate_1
|
| Data processing |
Raw data were processed with the Expression Console (Affymetrix) for background correction and normalization, and both probeset (exon) and gene (transcript cluster) level data were generated.
|
| |
|
| Submission date |
Mar 06, 2015 |
| Last update date |
Sep 28, 2015 |
| Contact name |
Jinsheng Yu |
| E-mail(s) |
jyu@wustl.edu
|
| Organization name |
Washington University School of Medicine
|
| Department |
Genetics
|
| Lab |
GTAC Lab
|
| Street address |
660 S. Euclid Ave.
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE66628 |
Multi-platform assessment of transcriptional profiling technologies utilizing a precise probe mapping methodology (Affymetrix_Gene1.0) (exon analysis) |
| GSE66649 |
Multi-platform assessment of transcriptional profiling technologies utilizing a precise probe mapping methodology |
|
| Relations |
| Alternative to |
GSM1626595 (exon-level analysis) |
