|
| Status |
Public on Mar 30, 2015 |
| Title |
siXrn2 treated cells 4h rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
A549 cells
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: Xrn2 siRNA
infection: A/PR/8/34(ΔNS8) Infection
cell line: A549
|
| Treatment protocol |
A549 cells were transfected with either control siRNA (D-001810-10-05, Dharmacon) or Xrn2 siRNA (L-017622-01-0010, Dharmacon) pools using the Lipofectamine™ RNAiMAX Transfection Reagent (Invitrogen). Transfections were performed according to the manufacturer’s instructions. 48 hours post transfection, the cells were infected with the Influenza A/Puerto Rico/8/34(ΔNS1) strain at an multiplicity of infection of 3. Wells of uninfected cells were used as controls for infection. Cells were collected for further processing 4 hours post infection. RNA and protein levels of Xrn2 were quantified to determine knockdown efficiency. Note: This experiment was done in parallel with the experiments described in GSE52933 and GSE52932 share the same UT and siCtrl treated controls, and hence share the same raw values for those two conditions
|
| Growth protocol |
Standard culture techniques
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QIAGEN Rneasy mini kit
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled RNA was prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems)
|
| |
|
| Hybridization protocol |
Standard Illumina protocol
|
| Scan protocol |
Standard Illumina protocol
|
| Description |
siXrn2 4h-2
|
| Data processing |
Data was analysed using Genespring GX12.5 . To analyze gene expression changes during stimulation, raw data were subject to quantile normalization and baseline transformation was performed to the median of unstimulated samples of the corresponding siRNA treatment.
|
| |
|
| Submission date |
Mar 09, 2015 |
| Last update date |
Mar 30, 2015 |
| Contact name |
Ivan Marazzi |
| Organization name |
Icahn School of Medicine at Mount Sinai
|
| Department |
Department of Microbiology
|
| Street address |
1468 Madison Avenue
|
| City |
New York |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE52937 |
Senataxin suppresses the antiviral transcriptional response and controls viral biogenesis |
| GSE66661 |
Analysis of global gene expression profiles of A549 cells treated with Xrn2 siRNA upon Influenza A/PR/8/34(ΔNS1) Infection |
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