|
Status |
Public on Apr 11, 2016 |
Title |
CEC_Donor E_passage 2_rep2 |
Sample type |
RNA |
|
|
Source name |
CEC, Donor E, passage 2
|
Organism |
Homo sapiens |
Characteristics |
cell type: corneal epithelium cells
|
Treatment protocol |
Retrovirus carring each TF was transduced to the fibroblasts.
|
Growth protocol |
All the cells were cultured in CEC medium which consists of Dulbecco’s modified Eagle’s medium and Ham’s F-12 media (1:1 mixture) (Life Technologies) containing B27-supplement (Life Technologies), Rho-kinase (ROCK) inhibitor (10 uM) (Nacalai Tesque), epidermal growth factor (10 ng/ml) (Life Technologies), Epigallocatechin gallate (SIGMA) and penicillin-streptomycin (50 IU/ml) (Nakalai Tesque),
|
Extracted molecule |
total RNA |
Extraction protocol |
Each sample was resuspended in a Qiagen lysis buffer, and RNA was prepared using the RNeasy Purification kit (Qiagen) following the manufacturer's recommendations.
|
Label |
biotin
|
Label protocol |
Standard Affymetrix protocol.
|
|
|
Hybridization protocol |
Standard Affymetrix protocol.
|
Scan protocol |
Standard Affymetrix protocol.
|
Data processing |
GeneSpring GX. Signals were normalized to percentile.
|
|
|
Submission date |
Apr 13, 2015 |
Last update date |
Apr 11, 2016 |
Contact name |
Koji Kitazawa |
E-mail(s) |
kkitazaw@koto.kpu-m.ac.jp
|
Organization name |
Kyoto Prefectural University of Medicine
|
Department |
Ophthalmology
|
Street address |
465 Kajiicho Kamigyoku
|
City |
Kyoto |
State/province |
Kyoto |
ZIP/Postal code |
601-0841 |
Country |
Japan |
|
|
Platform ID |
GPL16686 |
Series (2) |
GSE67820 |
Master transcription factors in corneal epithelial cells [6TFs transduced experimental samples] |
GSE67823 |
Master transcription factors in corneal epithelial cells |
|