|
| Status |
Public on Apr 11, 2016 |
| Title |
Fibroblasts_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Fibroblasts
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: fibroblasts
|
| Treatment protocol |
Retrovirus carring each TF was transduced to the fibroblasts.
|
| Growth protocol |
All the cells were cultured in CEC medium which consists of Dulbecco’s modified Eagle’s medium and Ham’s F-12 media (1:1 mixture) (Life Technologies) containing B27-supplement (Life Technologies), Rho-kinase (ROCK) inhibitor (10 uM) (Nacalai Tesque), epidermal growth factor (10 ng/ml) (Life Technologies), Epigallocatechin gallate (SIGMA) and penicillin-streptomycin (50 IU/ml) (Nakalai Tesque),
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Each sample was resuspended in a Qiagen lysis buffer, and RNA was prepared using the RNeasy Purification kit (Qiagen) following the manufacturer's recommendations.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol.
|
| Scan protocol |
Standard Affymetrix protocol.
|
| Data processing |
GeneSpring GX. Signals were normalized to percentile.
|
| |
|
| Submission date |
Apr 13, 2015 |
| Last update date |
Apr 11, 2016 |
| Contact name |
Koji Kitazawa |
| E-mail(s) |
kkitazaw@koto.kpu-m.ac.jp
|
| Organization name |
Kyoto Prefectural University of Medicine
|
| Department |
Ophthalmology
|
| Street address |
465 Kajiicho Kamigyoku
|
| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
601-0841 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16686 |
| Series (2) |
| GSE67820 |
Master transcription factors in corneal epithelial cells [6TFs transduced experimental samples] |
| GSE67823 |
Master transcription factors in corneal epithelial cells |
|
