|
| Status |
Public on Apr 11, 2016 |
| Title |
corneal epithlium_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
peripheral corneal epithelium, replicate 1
|
| Organism |
Homo sapiens |
| Characteristics |
pathology: Normal
cell type: peripheral corneal epithelium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Human CECs and conjunctival epithelial cells were isolated from donor cornea, conjunctival tissues via the treatment of 1000 protease units (PU)/ml of dispase at 4ºC overnight and TrypLE Express (Life Technologies) at 4ºC for 30 minutes. Each sample was resuspended in a Qiagen lysis buffer, and RNA was prepared using the RNeasy Purification kit (Qiagen) following the manufacturer's recommendations.
|
| Label |
Cy3
|
| Label protocol |
Manufacturer’s recommended condition.
|
| |
|
| Hybridization protocol |
Manufacturer’s recommended condition.
|
| Scan protocol |
Manufacturer’s recommended condition.
|
| Data processing |
GeneSpring GX. Signals were normalized to percentile.
|
| |
|
| Submission date |
Apr 13, 2015 |
| Last update date |
Apr 11, 2016 |
| Contact name |
Koji Kitazawa |
| E-mail(s) |
kkitazaw@koto.kpu-m.ac.jp
|
| Organization name |
Kyoto Prefectural University of Medicine
|
| Department |
Ophthalmology
|
| Street address |
465 Kajiicho Kamigyoku
|
| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
601-0841 |
| Country |
Japan |
| |
|
| Platform ID |
GPL14550 |
| Series (2) |
| GSE67822 |
Master transcription factors in corneal epithelial cells [in vivo samples] |
| GSE67823 |
Master transcription factors in corneal epithelial cells |
|
