|
| Status |
Public on May 25, 2015 |
| Title |
Microglia_germfree_4d_after_LCMV_ic_02 |
| Sample type |
RNA |
| |
|
| Source name |
Microglia, LCMV i.c., GF
|
| Organism |
Mus musculus |
| Characteristics |
treatment: LCMV
condition: germ free (GF)
cell type: microglia
|
| Treatment protocol |
Lymphocytic Choriomeningitis Virus (LCMV) strain WE was propagated and titrated as plaque forming units (PFU) on L929 cells as described before 12. PFU were multiplied by the factor 10 to be converted into infectious units (IU). Mice were infected by intracerebral inoculation of 103 IU into the right hemisphere
|
| Growth protocol |
Mice were housed under SPF or germ-free condiotions. Animals were 6-10 weeks old.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Adult microglia were harvested using density gradient separation. CD11b+CD45lo microglial cell populations were FACS-sorted and collected directly in cell lysis buffer and subsequently RNA was isolated with the Arcturus Pico Pure RNA Isolation Kit (Life Technologies) according to the manufacturer's protocol.
|
| Label |
Biotin
|
| Label protocol |
Total RNA was amplified using the Ovation PicoSL WTA System V2 and further processed using the Encore Biotin Module according to the instructions supplied by the manufacturer (NuGen).
|
| |
|
| Hybridization protocol |
Biotinylated cRNA was hybridized on GeneChip® Mouse Gene 2.1 ST Arrays , that were stained, washed and scanned following standard procedures.
|
| Scan protocol |
Biotinylated cRNA was hybridized on GeneChip® Mouse Gene 2.1 ST Arrays , that were stained, washed and scanned following standard procedures.
|
| Description |
Microglia from germ free (GF) mice treated with LCMV i.c.
|
| Data processing |
Background correction and normalization was performed using the RMA-Sketch implementation in Affymetrix Expression Console
|
| |
|
| Submission date |
Apr 14, 2015 |
| Last update date |
May 25, 2015 |
| Contact name |
Ori Staszewski |
| E-mail(s) |
ori.staszewski@uniklinik-freiburg.de
|
| Organization name |
University Medical Center Freiburg
|
| Department |
Institute of Neuropathology
|
| Street address |
Breisacher Str. 64
|
| City |
Freiburg |
| ZIP/Postal code |
D-79106 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17400 |
| Series (2) |
| GSE67857 |
Host microbiota constantly control maturation and function of microglia in the central nervous system (part 2) |
| GSE67858 |
Host microbiota constantly control maturation and function of microglia in the central nervous system |
|
