|
| Status |
Public on May 08, 2015 |
| Title |
control flies, ZT15 |
| Sample type |
RNA |
| |
|
| Source name |
control flies, ZT15
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: fly heads
treatment: control
|
| Growth protocol |
Flies were entrained in LD (light: dark) condition for 3-4 days and harvested at two time points: ZT3 and ZT15.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared (30 heads per sample) using Trizol reagent (Invitrogen) according to the manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
RNA was then used to prepare a probe that was hybridized to Drosophila 2.0 gene expression arrays (Affymetrix) according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Data processing |
CEL files were imported to Affymetrix Expression Console program, and data were normalized using Robust Multichips Analysis. Differential expression was tested for probes that had expression levels above 4.0 (log2 scale) in at least one group (mutants or WT). All other probes were excluded from the analysis.
|
| |
|
| Submission date |
Apr 14, 2015 |
| Last update date |
May 08, 2015 |
| Contact name |
Sebastian Kadener |
| E-mail(s) |
skadener@gmail.com
|
| Organization name |
Brandeis University
|
| Street address |
415 South Street
|
| City |
Waltham |
| State/province |
MA |
| ZIP/Postal code |
9190401 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE67865 |
Microarray data obtained from control, cbtRNAi and cbtOE flies |
|
