|
| Status |
Public on Apr 18, 2015 |
| Title |
Th17_invivo_GFPsorted_rep45 |
| Sample type |
RNA |
| |
|
| Source name |
in vivo Th17 cells GFP sorted
|
| Organism |
Mus musculus |
| Characteristics |
cell type: in vivo differentiated Th17
genotype: OT-II IL17GFP knock-in
|
| Treatment protocol |
None
|
| Growth protocol |
cells derived from OT-II IL17GFP knock-in were differentiated into Th17 in vitro by culturing splenocytes with IL-1b (10 ng/mL), IL-6 (10 ng/mL), IL-23 (10 ng/mL), human TGF-b (2.5 ng/mL) for 4 days and OVA 323-339 peptide or by in vivo exposure to Freund’s Adjuvant and OVA 323-339 peptide for 7 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with RNease Plus Mini Kit (Qiagen) and samples were processed and analyzed at the University of Michigan microarray facility with Affimetrix mouse Gene 1.0 ST array.
|
| Label |
biotin
|
| Label protocol |
cDNA were prepared according to the NuGen WT-Pico V2 kit protocol from 50 ng total RNA (Ovation PicoSL WTA System V2 P/N 3312). Biotinylated single-stranded cDNA were prepared from 3ug of cDNA (Encore Biotin Module P/N 4200-12, 4200-60, 4200-A01)
|
| |
|
| Hybridization protocol |
Following fragmentation, 3.7 ug of cDNA were hybridized for 20 hr at 48C on House Gene ST 2.1 strip arrays using the Affymetrix Gene Atlas system (software version 1.0.4.267).
|
| Scan protocol |
Arrays were scanned using the Affymetrix Gene Atlas system.
|
| Description |
Gene expression data from in vivo Th17 cells
|
| Data processing |
Data was analyzed using the oligo Bioconductor package implemented in the R-statistical environment. RMA was used to fit log2 transformed expression values based on a larger set of samples, which have not been deposited.
|
| |
|
| Submission date |
Apr 17, 2015 |
| Last update date |
Apr 18, 2015 |
| Contact name |
Gary Glick |
| Organization name |
Lycera
|
| Street address |
2800 Plymouth Road
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE68017 |
Expression data from in vitro versus in vivo differentiated Th17 cells |
|
