|
| Status |
Public on Apr 24, 2015 |
| Title |
primary neurons, day 28 |
| Sample type |
SRA |
| |
|
| Source name |
CD1 mouse embryos (E17.5–E18.5)
|
| Organism |
Mus musculus |
| Characteristics |
differentiation stage: cortical neurons, day 28 after plating
|
| Growth protocol |
Neurobasal-A medium with 2% B27 and 0.5 mM L-glutamine at 37ºC and 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIZOL
Ribodepletion followed by default Illumina RNA-Seq for HiSeq2500 (TruSeq)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
cortical neurons, day 28 after plating
genome build: mm9
|
| Data processing |
Illumina base calling, demultiplexing, and FASTQ conversion with CASAVA-1.8.2
alignment genome reference using bowtie2
20nt anchors from both ends of unaligned reads aligned to genome independently
aligned anchors were extended to find circRNA head-to-tail splice sites (see Suppl. Methods)
Supplementary_files_format_and_content: bed file; circRNA head-to-tail splice sites with number of supporting reads in the score column
|
| |
|
| Submission date |
Apr 23, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Petar Glazar |
| Organization name |
Max Delbrück Center for Molecular Medicine
|
| Department |
Systems Biology of Gene Regulatory Elements
|
| Lab |
Rajewsky lab
|
| Street address |
Robert Rössle Str. 10 (H. 87)
|
| City |
Berlin |
| ZIP/Postal code |
13092 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE65926 |
Circular RNAs in the mammalian brain are highly abundant, conserved, and dynamically expressed |
|
| Relations |
| BioSample |
SAMN03566480 |
| SRA |
SRX1007593 |
