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Sample GSM1684099 Query DataSets for GSM1684099
Status Public on Feb 01, 2016
Title Donor 3 - No virus control - 8h
Sample type RNA
 
Source name Blood pDCs
Organism Homo sapiens
Characteristics donor: Donor 3
agent: No virus control
cell type: Primary human pDCs
Treatment protocol pDCs were cultured with or without influenza A virus for 8 h.
Growth protocol Primary human pDCs were cultured in complete RPMI media (10% FBS, 1% penicillin-streptomycin, 1% sodium pyruvate, 1% HEPES buffer solution, 1% non-essential amino acids, 1% glutamate) and IL-3.
Extracted molecule total RNA
Extraction protocol RNA was extracted using Arcturus® PicoPure® RNA isolation kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Donor 3 - No virus control - 8h
Data processing The data were normalized using quantile normalization with Illumina Genome Studio
 
Submission date May 13, 2015
Last update date Feb 01, 2016
Contact name Michelle Ann Gill
Organization name University of Texas Southwestern Medical Center
Department Pediatrics
Street address 5323 Harry Hines Blvd
City Dallas
ZIP/Postal code 75390
Country USA
 
Platform ID GPL10558
Series (1)
GSE68849 Impact of influenza A on human plasmacytoid dendritic cells (pDC) gene expression

Data table header descriptions
ID_REF
VALUE Quantile normalized
5455178010_I.Avg_NBEADS
5455178010_I.BEAD_STDERR
5455178010_I.Detection Pval

Data table
ID_REF VALUE 5455178010_I.Avg_NBEADS 5455178010_I.BEAD_STDERR 5455178010_I.Detection Pval
ILMN_1762337 105.1461 30 4.957191 0.374026
ILMN_2055271 129.7449 18 7.727149 0.01948052
ILMN_1736007 113.549 18 8.353218 0.1662338
ILMN_2383229 107.4284 29 5.062422 0.3025974
ILMN_1806310 99.43924 21 6.865705 0.5545455
ILMN_1779670 78.92412 15 4.145588 0.9805195
ILMN_1653355 110.5088 15 9.208381 0.2363636
ILMN_1717783 81.72933 23 3.3602 0.9597403
ILMN_1705025 104.8214 18 6.707081 0.3805195
ILMN_1814316 103.6995 15 6.149187 0.4103896
ILMN_2359168 90.82912 23 5.861676 0.7844156
ILMN_1731507 89.85105 24 5.501346 0.8038961
ILMN_1787689 99.51279 25 6.256368 0.5493507
ILMN_3241953 125.2613 26 7.871883 0.03376623
ILMN_1745607 171.6573 23 10.02979 0
ILMN_2136495 85.78777 22 4.090424 0.8948052
ILMN_1668111 100.5574 17 4.505511 0.5194805
ILMN_2295559 111.3235 21 8.795664 0.2142857
ILMN_1735045 203.1339 30 9.12153 0
ILMN_1680754 111.2953 13 11.63041 0.2142857

Total number of rows: 47321

Table truncated, full table size 1944 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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