|
Status |
Public on Feb 01, 2016 |
Title |
Donor 3 - No virus control - 8h |
Sample type |
RNA |
|
|
Source name |
Blood pDCs
|
Organism |
Homo sapiens |
Characteristics |
donor: Donor 3 agent: No virus control cell type: Primary human pDCs
|
Treatment protocol |
pDCs were cultured with or without influenza A virus for 8 h.
|
Growth protocol |
Primary human pDCs were cultured in complete RPMI media (10% FBS, 1% penicillin-streptomycin, 1% sodium pyruvate, 1% HEPES buffer solution, 1% non-essential amino acids, 1% glutamate) and IL-3.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using Arcturus® PicoPure® RNA isolation kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
Donor 3 - No virus control - 8h
|
Data processing |
The data were normalized using quantile normalization with Illumina Genome Studio
|
|
|
Submission date |
May 13, 2015 |
Last update date |
Feb 01, 2016 |
Contact name |
Michelle Ann Gill |
Organization name |
University of Texas Southwestern Medical Center
|
Department |
Pediatrics
|
Street address |
5323 Harry Hines Blvd
|
City |
Dallas |
ZIP/Postal code |
75390 |
Country |
USA |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE68849 |
Impact of influenza A on human plasmacytoid dendritic cells (pDC) gene expression |
|