|
| Status |
Public on Feb 01, 2016 |
| Title |
Donor 3 - Influenza treated - 8h |
| Sample type |
RNA |
| |
|
| Source name |
Blood pDCs
|
| Organism |
Homo sapiens |
| Characteristics |
donor: Donor 3
agent: Influenza A
cell type: Primary human pDCs
|
| Treatment protocol |
pDCs were cultured with or without influenza A virus for 8 h.
|
| Growth protocol |
Primary human pDCs were cultured in complete RPMI media (10% FBS, 1% penicillin-streptomycin, 1% sodium pyruvate, 1% HEPES buffer solution, 1% non-essential amino acids, 1% glutamate) and IL-3.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Arcturus® PicoPure® RNA isolation kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Donor 3 - Influenza treated - 8h
|
| Data processing |
The data were normalized using quantile normalization with Illumina Genome Studio
|
| |
|
| Submission date |
May 13, 2015 |
| Last update date |
Feb 01, 2016 |
| Contact name |
Michelle Ann Gill |
| Organization name |
University of Texas Southwestern Medical Center
|
| Department |
Pediatrics
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE68849 |
Impact of influenza A on human plasmacytoid dendritic cells (pDC) gene expression |
|
