|
| Status |
Public on Jul 01, 2015 |
| Title |
Embryonic day 17.5 (E17.5) Nasal Septum, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Embryonic day 17.5_Nasal Septum
|
| Organism |
Mus musculus |
| Characteristics |
strain: ICR
developmental stage/age: embryonic day 17.5
cell type: Nasal chondrocyte
|
| Treatment protocol |
Wild type, not treated
|
| Growth protocol |
Neonatal ICR wild-type mice embryonic day 17.5 nasal septum or postnatal day 1 rib
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Embryonic day 17.5 (E17.5) nasal septum or postnatal day 1 (p1) rib chondrocytes isolated from wild-type ICR mice were subject to Trizol total RNA isolation. Isolated RNAs were digested with DNAse I.
|
| Label |
Biotin
|
| Label protocol |
Three hundred ng of total RNA were labeled for each sample using the GeneChip WT cDNA Synthesis and Amplification Kit and WT Terminal Labeling Kit (900673 and 900671; Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 25ul of cDNA were hybridized for 16 hr at 45C on GeneChip Mouse Gene Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Chips were scanned with GeneChip Scanner 3000 (Affymetrix) to generate image and CEL files, using Affymetrix default analysis settings and global scaling as normalization method
|
| Description |
NasalCh_REP3.CEL
|
| Data processing |
Data analysis was performed by loading CEL files into the statistical computing language R software, and probes were normalized using robust multi-array average (RMA) (CDF: MoGene10stv1_Mm_REFSEQ_14.1.0)
|
| |
|
| Submission date |
May 21, 2015 |
| Last update date |
Jul 01, 2015 |
| Contact name |
Xinjun He |
| E-mail(s) |
xinjun.he@med.usc.edu
|
| Phone |
323-442-8077
|
| Organization name |
University of Southern California
|
| Department |
BROAD CIRM Center
|
| Street address |
1425 San Pablo St
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
|
| Platform ID |
GPL20205 |
| Series (2) |
| GSE69108 |
Distinct regulatory programs for Sox9 in transcriptional regulation of the developing mammalian chondrocyte [expression array] |
| GSE69111 |
Distinct regulatory programs for Sox9 in transcriptional regulation of the developing mammalian chondrocyte |
|
