|
| Status |
Public on Jun 03, 2015 |
| Title |
TE-5R |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
TE-5R
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: TE-5R (5-FU-resistant subline)
|
| Treatment protocol |
TE-5 cells were treated with continuous and step-wise concentrations of 5-FU (1, 2, 5, and 10 μM), and consequently 5-FU-resistant ESCC cells were established and named as TE-5R cells.
|
| Growth protocol |
TE-5 and TE-5R cells were cultured in RPMI1640 medium supplemented with 10% fetal bovine serum, 100 μg/ml streptomycin and 100 units/ml penicillin at 37 ºC in a 5% CO2 incubator.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from TE-5 and TE-5R cells and labelled according to Oligonucleotide Array-Based CGH for Genomic DNA Analysis Protocol.
|
| Label |
Cy5
|
| Label protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| |
|
| Channel 2 |
| Source name |
Control
|
| Organism |
Homo sapiens |
| Characteristics |
reference: Human Reference DNA Female
|
| Treatment protocol |
TE-5 cells were treated with continuous and step-wise concentrations of 5-FU (1, 2, 5, and 10 μM), and consequently 5-FU-resistant ESCC cells were established and named as TE-5R cells.
|
| Growth protocol |
TE-5 and TE-5R cells were cultured in RPMI1640 medium supplemented with 10% fetal bovine serum, 100 μg/ml streptomycin and 100 units/ml penicillin at 37 ºC in a 5% CO2 incubator.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from TE-5 and TE-5R cells and labelled according to Oligonucleotide Array-Based CGH for Genomic DNA Analysis Protocol.
|
| Label |
Cy3
|
| Label protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| |
|
| |
| Hybridization protocol |
Scanned on an Agilent SureScan scanner.
|
| Scan protocol |
Images were quantified using Agilent Feature Extraction Software
|
| Data processing |
Agilent Feature Extraction Software (v 8.5.1.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Jun 03, 2015 |
| Last update date |
Jun 03, 2015 |
| Contact name |
Osamu Kikuchi |
| Organization name |
Kyoto University
|
| Department |
Therapeutic Oncology
|
| Street address |
54 Shogoin-Kawaharacho Sakyoku
|
| City |
Kyoto |
| ZIP/Postal code |
6068507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL19387 |
| Series (1) |
|
