|
| Status |
Public on Mar 02, 2016 |
| Title |
72h rep1 |
| Sample type |
SRA |
| |
|
| Source name |
adenocarcinomic human alveolar basal epithelial cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
cell type: adenocarcinomic human alveolar basal epithelial
time point: 72h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent.
RNA libraries were prepared for sequencing using standard Illumina protocols, and Illumina TruSeq RNA Sample Prep Kit was used for library construction.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
TGF-beta induced EMT
|
| Data processing |
Reads were mapped to ENSEMBL release 75 transcripts by Bowtie, then TPM values were calculated by RSEM to characterize gene expression profiles.
|
| |
|
| Submission date |
Jun 08, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Mengzhu Xue |
| E-mail(s) |
xuemz@sari.ac.cn
|
| Organization name |
Shanghai Advanced Research Institute
|
| Street address |
No.100, Haike Road
|
| City |
Shanghai |
| ZIP/Postal code |
201210 |
| Country |
China |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE69667 |
A systematic analysis of the time series gene expression in TGF-beta induced EMT by Next-generation sequencing |
|
| Relations |
| BioSample |
SAMN03765214 |
| SRA |
SRX1053760 |
