|
| Status |
Public on Jun 12, 2016 |
| Title |
Patient4_T |
| Sample type |
SRA |
| |
|
| Source name |
Peripheral blood mononuclear cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: sorted CD8 pos CD57 pos T cells
sample type: tumour (T-cell large granular lymphocytic leukemia)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Peripheral blood mononuclear were stained with anti CD8 PE and CD57 FITC antibodies and sorted by FACS. RNA was extracted with TRIzol and purified with the RNeasy Mini Kit
Libraries were prepared using the NuGEN Encore platform according to manufacturer´s giudelines
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Base calling with CASAVA 1.8.2
Alignment was performed with TopHat2
For quantification and variant calling Avadis NGS (Strand) was used.
Genome_build: hg19
|
| |
|
| Submission date |
Jun 10, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Ludger Klein-Hitpass |
| E-mail(s) |
ludger.klein-hitpass@uni-essen.de
|
| Phone |
+49 201 723 85552
|
| Organization name |
Institut fuer Zellbiologie
|
| Department |
Universitaetsklinikum
|
| Lab |
BioChip Lab
|
| Street address |
Virchowstr. 173
|
| City |
Essen |
| ZIP/Postal code |
D-45122 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE69735 |
Recurrent alterations of TNFAIP3 (A20) in T-cell large granular lymphocytic leukemia |
|
| Relations |
| BioSample |
SAMN03768377 |
| SRA |
SRX1055058 |
