|
Status |
Public on Mar 01, 2007 |
Title |
H3K18Ac HeLa ENCODE NimbleGen hg17 tiling array replicate 1 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
Chromatin immunoprecipitated DNA from HeLa cells using H3K18Ac antibody
|
Organism |
Homo sapiens |
Characteristics |
cells: HeLa immunoprecipitated chromatin
|
Extracted molecule |
genomic DNA |
Extraction protocol |
immunoprecipitated chromatin
|
Label |
Cy5
|
Label protocol |
Cy5
|
|
|
Channel 2 |
Source name |
Input Chromatin HeLa cells.
|
Organism |
Homo sapiens |
Characteristics |
cells: HeLa input chromatin
|
Extracted molecule |
genomic DNA |
Extraction protocol |
input chromatin
|
Label |
Cy3
|
Label protocol |
Cy3
|
|
|
|
Hybridization protocol |
NimbleGen hybridization protocol
|
Scan protocol |
NimbleGen scan protocol
|
Description |
Chromatin immunoprecipitation was performed using antibodies against H3K18Ac and formaldehyde crosslinked chromatin from HeLa cells. The immunoprecipitated DNA was amplified, fluorescently labeled and hybridized to a 391K element tiling array based on NCBI35 build of the human genome (hg17) that contains overlapping 50-mer oligonucleotides, positioned at every 38 basepairs (bp) throughout the ENCODE regions.
|
Data processing |
raw data
|
|
|
Submission date |
Feb 24, 2007 |
Last update date |
Feb 26, 2007 |
Contact name |
Bing Ren |
E-mail(s) |
biren@ucsd.edu
|
Organization name |
Ludwig Institute for Cancer Research
|
Department |
Department of Cellular and Molecular Medicine
|
Lab |
Laboratory of Gene Regulation
|
Street address |
9500 Gilman Drive
|
City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92093-0653 |
Country |
USA |
|
|
Platform ID |
GPL4559 |
Series (1) |
GSE7118 |
Ludwig Institute/UCSD ENCODE project March 2007 Release |
|