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Status |
Public on Nov 30, 2016 |
Title |
GEN2.2-0 hrs post CpGB-MYC siRNA-rep 3 |
Sample type |
RNA |
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|
Source name |
GEN2.2-0 hrs post CpGB-MYC siRNA
|
Organism |
Homo sapiens |
Characteristics |
cell line: GEN2.2 hrs post cpgb stimulation: 0 sirna: MYC replicate: 3
|
Treatment protocol |
GEN2.2 cells were myc-ablated by Nucleofection (Nucleofector kit V, www.lonza.com). GEN2.2 cells were nucleofected with nucleofector kit V (Amaxa) with 0.4 nmol of siRNA for each reaction of 3-5 × 10^6 cells. siMYC and siHDAC3 were purchased from Dharmacon. The program number for electroporation was A033. The non-targeting control (NC) is a non-targeting pool from Dharmacon. GEN2.2 cells were stimulated with 0.5 µM of CpGB
|
Growth protocol |
GEN2.2 cells were kindly provided by Dr. Joël Plumas (Université Joseph Fourier, Grenoble, France) and cultured in GlutaMax-RPMI (GIBCO) supplemented with 10% FBS (Atlanta), MEM-nonessential amino acid solution (GIBCO), sodium pyruvate and gentamycin.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA isolation was performed using Qiagen RNeasy mini kit according to the manufacturer’s instructions
|
Label |
biotin
|
Label protocol |
Standard Illumina labelling protocol.
|
|
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Hybridization protocol |
Standard Illumina hybridization protocol.
|
Scan protocol |
Standard Illumina scanning protocol.
|
Description |
9373549130_J
|
Data processing |
Standard Average Normalization as detailed by GenomeStudio
|
|
|
Submission date |
Jun 25, 2015 |
Last update date |
Mar 16, 2023 |
Contact name |
Nicole Baldwin |
E-mail(s) |
Nicole.Baldwin@BSWHealth.org
|
Organization name |
Baylor Research Institute
|
Street address |
3434 Live Oak St
|
City |
Dallas |
ZIP/Postal code |
75204 |
Country |
USA |
|
|
Platform ID |
GPL10558 |
Series (2) |
GSE70276 |
Transcriptional repression of IRF7 by MYC is critical for antiviral immune response in human pDC [GEN2.2] |
GSE70278 |
Transcriptional repression of IRF7 by MYC is critical for antiviral immune response in human pDC |
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