The biopsies were individually homogenised in Lysis Binding buffer (100mM Tris-HCl, pH7.5, 500mM LiCl, 10mM EDTA, pH 8.0, 1% LiDS, 5mM dithiothreitol). Long SAGE libraries were constructed according to the L-SAGE kit manual (Invitrogen, Ontario, Canada). Sequencing was performed at the BC Cancer Agency Michael Smith Genome Sciences Centre.
Description
The specimens were collected immediately prior to the LEEP (Loop electrosurgical excision procedure) cone biopsy targeting a small portion of the affected epithelium. These specimens were collected with patient consent at the Vancouver General Hospital Women’s Clinic at Vancouver Hospital & Health Science Centre.
