|
| Status |
Public on Dec 10, 2015 |
| Title |
Neurons_siRbfox1&3_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Hippocampal culture, AraC treated, co-cultured, Rbfox1 and Rbfox3 siRNAs
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: Hippocampal culture
age: 14 DIV
treatment: AraC and co-culture
sirna: Rbfox1 and Rbfox3
|
| Treatment protocol |
Cultures were treated with AraC at 3 DIV and co-cultured with cortical culture at 6 DIV. SiRNAs were added at 10 DIV and incubated for 4 days.
|
| Growth protocol |
The mouse neurons were cultured as decribed by Ho et al. (PMID: 24784359).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by RNeasy Mini kit (Qiagen) according to manufacturer's instruction.
|
| Label |
biotin
|
| Label protocol |
Samples were labeled using GeneChip Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridization was performed as specified, with the GeneChip Hybridization Wash and Stain Kit (Affymetrix).
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G.
|
| Description |
Hippocampal culture treated AraC, co-culture, and siRNAs
siRbfox1&3_Rep1
|
| Data processing |
Affymetrix package (Affy Power Tools) apt-probeset-summarize. Iter-plier algorithm used to quantify probesets.
probe group file: mjay.pgf
meta-probeset file: mjay.clf
|
| |
|
| Submission date |
Aug 12, 2015 |
| Last update date |
Dec 10, 2015 |
| Contact name |
Chia-ho Lin |
| E-mail(s) |
CHIAHOLI@g.UCLA.EDU
|
| Organization name |
UCLA
|
| Lab |
BLACK LAB
|
| Street address |
6577 MRL. 675 CHARLES E YOUNG DR. SOUTH
|
| City |
LOS ANGELES |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL13185 |
| Series (2) |
| GSE71917 |
Cytoplasmic Rbfox1 regulates the neuronal expression of synaptic and autism-related genes |
| GSE71974 |
Gene expression profiling of neurons with Rbfox1 and Rbfox3 knockdown |
|
