|
Status |
Public on Jul 28, 2016 |
Title |
EM3 |
Sample type |
RNA |
|
|
Source name |
embryonic microglia
|
Organism |
Mus musculus |
Characteristics |
tissue: brain dev.stage: E14 celltype: microglia genotype: wildtype
|
Treatment protocol |
samples from liver, kidney and skin were treated with collagenase. Skin and Liver was additionaly treated with dispase and DNase for digesting the tissue. Brain cells (microglia) were isolated by a percoll-gradient.
|
Growth protocol |
isolated cells
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA isolation was perfomed using Arcturus PicoPure isolation kit according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Total RNA was amplified using the Ovation PicoSL WTA System V2 and further processed using the Encore Biotin Module according to the instructions supplied by the manufacturer (NuGen).
|
|
|
Hybridization protocol |
Biotinylated cRNA was hybridized on GeneChip® Mouse Gene 2.1 ST Arrays , that were stained, washed and scanned following standard procedures.
|
Scan protocol |
Biotinylated cRNA was hybridized on GeneChip® Mouse Gene 2.1 ST Arrays , that were stained, washed and scanned following standard procedures.
|
Description |
embryonic microglia wildtype rep3
|
Data processing |
Background correction and normalization was performed using the RMA-Sketch implementation in Affymetrix Expression Console
|
|
|
Submission date |
Sep 17, 2015 |
Last update date |
Jul 28, 2016 |
Contact name |
Ori Staszewski |
E-mail(s) |
ori.staszewski@uniklinik-freiburg.de
|
Organization name |
University Medical Center Freiburg
|
Department |
Institute of Neuropathology
|
Street address |
Breisacher Str. 64
|
City |
Freiburg |
ZIP/Postal code |
D-79106 |
Country |
Germany |
|
|
Platform ID |
GPL17400 |
Series (1) |
GSE73125 |
Transcriptome-based profiling reveals a macrophage pedigree and identifies Irf8 as pivotal for macrophage homeostasis and function |
|