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Sample GSM1887444 Query DataSets for GSM1887444
Status Public on Jul 28, 2016
Title eliv-R4-ko3_2
Sample type RNA
 
Source name embryonic CD11bhigh macrophages
Organism Mus musculus
Characteristics tissue: liver
dev.stage: E14
celltype: macrophages
genotype: Irf8 knockout
Treatment protocol samples from liver, kidney and skin were treated with collagenase. Skin and Liver was additionaly treated with dispase and DNase for digesting the tissue. Brain cells (microglia) were isolated by a percoll-gradient.
Growth protocol isolated cells
Extracted molecule total RNA
Extraction protocol RNA isolation was perfomed using Arcturus PicoPure isolation kit according to the manufacturer's instructions.
Label biotin
Label protocol Total RNA was amplified using the Ovation PicoSL WTA System V2 and further processed using the Encore Biotin Module according to the instructions supplied by the manufacturer (NuGen).
 
Hybridization protocol Biotinylated cRNA was hybridized on GeneChip® Mouse Gene 2.1 ST Arrays , that were stained, washed and scanned following standard procedures.
Scan protocol Biotinylated cRNA was hybridized on GeneChip® Mouse Gene 2.1 ST Arrays , that were stained, washed and scanned following standard procedures.
Description embryonic liver CD11bhigh macrophages Irf8KO rep2
Data processing Background correction and normalization was performed using the RMA-Sketch implementation in Affymetrix Expression Console
 
Submission date Sep 17, 2015
Last update date Jul 28, 2016
Contact name Ori Staszewski
E-mail(s) ori.staszewski@uniklinik-freiburg.de
Organization name University Medical Center Freiburg
Department Institute of Neuropathology
Street address Breisacher Str. 64
City Freiburg
ZIP/Postal code D-79106
Country Germany
 
Platform ID GPL17400
Series (1)
GSE73125 Transcriptome-based profiling reveals a macrophage pedigree and identifies Irf8 as pivotal for macrophage homeostasis and function

Data table header descriptions
ID_REF
VALUE Log2 RMA

Data table
ID_REF VALUE
17200001 3.7074
17200003 4.30627
17200005 3.22565
17200007 3.54812
17200009 2.91263
17200011 3.81803
17200013 3.05833
17200015 4.27969
17200017 3.62675
17200019 3.97494
17200021 1.15744
17200023 4.00217
17200025 2.3564
17200027 3.78837
17200029 1.75698
17200031 3.16623
17200033 4.16375
17200035 3.94315
17200037 3.86955
17200039 0.746051

Total number of rows: 41345

Table truncated, full table size 682 Kbytes.




Supplementary file Size Download File type/resource
GSM1887444_A912c_08_eliv-R4-ko3_2.CEL.gz 4.8 Mb (ftp)(http) CEL
GSM1887444_A912c_08_eliv-R4-ko3_2.rma-gene-full.chp.gz 258.6 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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