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Sample GSM1888236 Query DataSets for GSM1888236
Status Public on Sep 25, 2015
Title cells treated with retinoic acid [RA1]
Sample type RNA
 
Source name NaF cells grown in charcoal-treated FBS (10%) DMEM and treated with all-trans retinoic acid (1uM) for 6 hours
Organism Mus musculus
Characteristics cell line: NaF
Treatment protocol Cells were treated with ligands for 6 hours
Growth protocol NaF cells were grown in full media (DMEM 10% FBS). 48 hours before ligand treatment media was replaced to DMEM containing 10% charcoal-treated FBS. Palmitate treatment was done in full media and samples were compared to full growth-media -grown control (samples C1-C3).
Extracted molecule total RNA
Extraction protocol RNA was extracted using Qiazol than cleaned using RNAeasy columns (Qiagene)
Label biotin
Label protocol Biotinylated-cRNA was synthesized form total RNA samples and labeled using the Affymetrix IVT labeling protocol.
 
Hybridization protocol Hybridization was done using the standard Affymetrix' WT labeling kit and protocol.
Scan protocol Hybridized arrays were automatically stained and scanned with the Affymetrix standard GeneTitan protocols.
Description Gene expression data from NaF cell in response to different PPARd and RAR treatments
Data processing data was processed using Partek-Genomics-Suite (PGS) v6.6 software (Qiagene).
 
Submission date Sep 18, 2015
Last update date Sep 25, 2015
Contact name Liraz Levi
E-mail(s) levil@ccf.org
Organization name Lerner Research Institute; Cleveland Clinic
Department Cellular and Molecular Medicine
Lab Noa Noy
Street address 9500 Euclid Avenue
City Cleveland
State/province OH
ZIP/Postal code 44195
Country USA
 
Platform ID GPL17400
Series (1)
GSE73186 Saturated fatty acids regulate retinoic acid signaling and suppress tumorigenesis by targeting fatty acid-binding protein 5

Data table header descriptions
ID_REF
VALUE RMA normalized log2 signal

Data table
ID_REF VALUE
17210850 0.7270247
17210852 0.7205928
17210855 7.040265
17210869 6.682066
17210883 0.653604
17210887 6.16641
17210904 0.7885172
17210912 5.580446
17210947 0.7965263
17210953 0.8129988
17210984 5.149637
17210994 0.6573031
17210996 0.9356124
17210998 0.6121895
17211000 4.270393
17211004 1.220375
17211023 0.7278329
17211033 0.8274814
17211043 3.761992
17211066 0.4984127

Total number of rows: 41345

Table truncated, full table size 740 Kbytes.




Supplementary file Size Download File type/resource
GSM1888236_R1.CEL.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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