|
| Status |
Public on Sep 25, 2015 |
| Title |
cells treated with PPARd agonist, GW1516 [GW1] |
| Sample type |
RNA |
| |
|
| Source name |
NaF cells grown in charcoal-treated FBS (10%) DMEM and treated with GW1516 (1uM) for 6 hours
|
| Organism |
Mus musculus |
| Characteristics |
cell line: NaF
|
| Treatment protocol |
Cells were treated with ligands for 6 hours
|
| Growth protocol |
NaF cells were grown in full media (DMEM 10% FBS). 48 hours before ligand treatment media was replaced to DMEM containing 10% charcoal-treated FBS. Palmitate treatment was done in full media and samples were compared to full growth-media -grown control (samples C1-C3).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Qiazol than cleaned using RNAeasy columns (Qiagene)
|
| Label |
biotin
|
| Label protocol |
Biotinylated-cRNA was synthesized form total RNA samples and labeled using the Affymetrix IVT labeling protocol.
|
| |
|
| Hybridization protocol |
Hybridization was done using the standard Affymetrix' WT labeling kit and protocol.
|
| Scan protocol |
Hybridized arrays were automatically stained and scanned with the Affymetrix standard GeneTitan protocols.
|
| Description |
Gene expression data from NaF cell in response to different PPARd and RAR treatments
|
| Data processing |
data was processed using Partek-Genomics-Suite (PGS) v6.6 software (Qiagene).
|
| |
|
| Submission date |
Sep 18, 2015 |
| Last update date |
Sep 25, 2015 |
| Contact name |
Liraz Levi |
| E-mail(s) |
levil@ccf.org
|
| Organization name |
Lerner Research Institute; Cleveland Clinic
|
| Department |
Cellular and Molecular Medicine
|
| Lab |
Noa Noy
|
| Street address |
9500 Euclid Avenue
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44195 |
| Country |
USA |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE73186 |
Saturated fatty acids regulate retinoic acid signaling and suppress tumorigenesis by targeting fatty acid-binding protein 5 |
|
