|
| Status |
Public on Apr 22, 2016 |
| Title |
iEP-derived red colonies, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse colonies derived from day 5-iEPs
|
| Organism |
Mus musculus |
| Characteristics |
assay: Colony assay
|
| Treatment protocol |
Cells were pelleted at 350g and resuspended in 350ul of RLT buffer (Qiagen) and frozen at -80°C.
|
| Growth protocol |
For colony-forming assays MethoCult M3236 (Stem Cell Technologies) was used and supplemented with 5U/mL rhEPO, 50ng/mL mSCF, 100nM dexamethasone and 1% antibiotics. Cells were resuspended in FCS and added to MethoCult mixture. Colonies were scored at day 8.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with RNAeasy micro kit (Qiagen) according to the manufacturers protocol.
|
| Label |
biotin
|
| Label protocol |
Affymetrix protocol
|
| |
|
| Hybridization protocol |
Samples were hybridised to the Affymetrix MoGene 2.1 array accroding to Affymetrix protocols
|
| Scan protocol |
Scanning was performed according to Affymetrix protocols
|
| Description |
Gene expression data from visibly red colonies derived from day 5-iEPs
iEP_red2
|
| Data processing |
Signal intensities from the MAS5 suite were RMA summarised
|
| |
|
| Submission date |
Sep 23, 2015 |
| Last update date |
Apr 23, 2016 |
| Contact name |
Shamit Soneji |
| Organization name |
BMC
|
| Street address |
Solvegatan 19
|
| City |
Lund |
| ZIP/Postal code |
22184 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE73344 |
Expression data from mouse induced Erythroid Progenitor (iEP)-derived colonies, mouse bone marrow and fetal liver BFU-Es and mouse fibroblasts. |
|
