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Status
Public on Feb 20, 2016
Title
B39_24hr_3
Sample type
RNA
Source name
hFOB
Organism
Homo sapiens
Characteristics
cell line: hFOB treatment: untreated, grow at 39oC in BM
Treatment protocol
BM, basal madia: DMEM supplemented 10% calf serum, 2mM L-glutamine, 1mM sodium pyruvate, 1X non-essential amino acids, and 1% penicillin-streptomycin
Growth protocol
BM, basal madia: DMEM supplemented 10% calf serum, 2mM L-glutamine, 1mM sodium pyruvate, 1X non-essential amino acids, and 1% penicillin-streptomycin
Extracted molecule
total RNA
Extraction protocol
none provided by submitter
Label
biotin
Label protocol
standard Affymetrix protocol
Hybridization protocol
standard Affymetrix protocol
Scan protocol
standard Affymetrix protocol
Description
B39_24hr_3
Data processing
Affymetrix human Genome U133 2.0 array data in CEL files were preprocessed using Affymetrix Expression Console software with mas5 and its quantile normalization method. Mean expression values from biological duplicates were calculated.
Submission date
Nov 20, 2015
Last update date
Feb 20, 2016
Contact name
Songjoon Baek
Organization name
NCI / NIH
Department
CCR
Lab
LRBGE
Street address
41 Library Drive
City
Bethesda
State/province
MD
ZIP/Postal code
20892
Country
USA
Platform ID
GPL570
Series (2)
GSE75230
Genome-Wide Chromatin Landscape Transitions Identify Novel Pathways in Early Commitment to Osteoblast Differentiation (microarray)
GSE75232
Genome-Wide Chromatin Landscape Transitions Identify Novel Pathways in Early Commitment to Osteoblast Differentiation
Data table header descriptions
ID_REF
VALUE
Signal
ABS_CALL
indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-5_at
428.015
P
5.16732e-05
AFFX-BioB-M_at
609.349
P
4.42873e-05
AFFX-BioB-3_at
358.105
P
4.42873e-05
AFFX-BioC-5_at
895.168
P
5.16732e-05
AFFX-BioC-3_at
1616.28
P
4.42873e-05
AFFX-BioDn-5_at
2545.28
P
4.42873e-05
AFFX-BioDn-3_at
3800.43
P
4.42873e-05
AFFX-CreX-5_at
11920.4
P
5.16732e-05
AFFX-CreX-3_at
12405
P
4.42873e-05
AFFX-DapX-5_at
202.551
P
6.02111e-05
AFFX-DapX-M_at
696.197
P
0.000169227
AFFX-DapX-3_at
1229.43
P
4.42873e-05
AFFX-LysX-5_at
64.671
P
0.00010954
AFFX-LysX-M_at
76.2405
P
0.00159257
AFFX-LysX-3_at
145.826
P
6.02111e-05
AFFX-PheX-5_at
90.8355
P
4.42873e-05
AFFX-PheX-M_at
132.51
P
7.00668e-05
AFFX-PheX-3_at
123.935
P
4.42873e-05
AFFX-ThrX-5_at
51.1083
P
0.0032123
AFFX-ThrX-M_at
106.011
P
0.000146402
Total number of rows: 54675 Table truncated, full table size 1639 Kbytes .Supplementary file
Size
Download
File type/resource
GSM1946420_B39_24hr_3.CEL.gz
4.5 Mb
(ftp) (http)
CEL
GSM1946420_B39_24hr_3.mas5.CHP.gz
488.7 Kb
(ftp) (http)
CHP
Processed data included within Sample table
Processed data provided as supplementary file