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Status |
Public on Aug 08, 2007 |
Title |
NA12750 growth 1, replicate 1 |
Sample type |
RNA |
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Source name |
CEU NA12750 Biological growth 1, Technical replicate 1
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Organism |
Homo sapiens |
Characteristics |
Gender: male, Cell-type: lymphoblasts Coriell cell line repository identifier: GM12750 http://locus.umdnj.edu/nigms/nigms_cgi/display.cgi?GM12750
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Biomaterial provider |
Coriell Cell Repositories http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=NA12750
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Treatment protocol |
Cells were harvested at a density of 0.8-1.1 x 10e6 cells/mL. Cells were then resuspended and lysed in TRIzol reagent
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Growth protocol |
Cells were grown at 37°C and 5% CO2 in RPMI 1640 medium supplemented with 15% heat-inactivated fetal bovine serum, 2 mM L-glutamine and penicillin/streptomycin
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
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Label |
biotin
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Label protocol |
Biotinlabelled target for the microarray experiment were prepared using 1 ug of total RNA. The RNA was subjected to a rRNA removal procedure with the RiboMinus Human/Mouse Transcriptome Isolation Kit (Invitrogen) and cDNA was synthesized using the GeneChip® WT (Whole Transcript) Sense Target Labeling and Control Reagents kit as described by the manufacturer (Affymetrix).
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Hybridization protocol |
Following fragmentation, 5 ug of cRNA were hybridized for 16-20 hr at 45C on GeneChip Human Exon 1.0 ST array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G (Affymetrix).
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Description |
Probeset expression data from NA12750 growth 1, replicate 1
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Data processing |
The data were analyzed with Affymetrix Power Tools package using Affymetrix default analysis settings and sketch quantile as normalization method.
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Submission date |
May 29, 2007 |
Last update date |
Apr 26, 2009 |
Contact name |
Tony Kwan |
E-mail(s) |
tony.kwan@mcgill.ca
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Phone |
514-398-3311
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Organization name |
McGill University
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Department |
Human Genetics
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Street address |
740 Dr. Penfield
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City |
Montreal |
State/province |
Quebec |
ZIP/Postal code |
H3A 0G1 |
Country |
Canada |
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Platform ID |
GPL5175 |
Series (1) |
GSE7952 |
Heritability of alternative splicing in the human genome |
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