|
| Status |
Public on Jan 07, 2016 |
| Title |
HT29tsp53-p53 off- 2 |
| Sample type |
RNA |
| |
|
| Source name |
HT29-tsp53 control at 38˚C
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HT29-tsp53
cell type: Human Colorectal Adenocarcinoma Cell Line
genotype/variation: expressing a temperature sensitive variant of p53 (murine V135A p53 transgene)
temperature (38 or 32˚c): 38˚C
|
| Treatment protocol |
One dish of subconfluent cells (60-70% confluent) were moved from the 37˚C incubator to a similarly regulated 32˚C incubator for 16 hours.
|
| Growth protocol |
Cells were grown as adherent cultures in 5% CO2 at 37˚C in a humidified incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Qiagen RNeasy Mini Kit according to the manufacturer's recommendations.
|
| Label |
biotin
|
| Label protocol |
100ng of total RNA was labeled with Whole Transcript (WT) Sense Target Labeling kit as specified by the manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
starndard Affymetrix protocol
|
| Scan protocol |
Gene Chips were scanned with Affymetrix Scanner 3000.
|
| Description |
expt 2 38
|
| Data processing |
FlexArray 1.4.1, APT
probe group file: HuGene-1_0-st-v1.r3.pgf
meta-probeset file: HuGene-1_0-st-v1.r3.mps
|
| |
|
| Submission date |
Jan 06, 2016 |
| Last update date |
Jan 07, 2016 |
| Contact name |
Bruce McKay |
| E-mail(s) |
Bruce_McKay@carleton.ca
|
| Phone |
6135202600
|
| Organization name |
Carleton University
|
| Department |
Biology
|
| Street address |
1125 Colonel By Dr.
|
| City |
Ottawa |
| State/province |
Ontario |
| ZIP/Postal code |
K1S 5B6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL10739 |
| Series (1) |
| GSE76575 |
The role of mRNA decay in p53-induced gene expression |
|
