|
| Status |
Public on Feb 16, 2016 |
| Title |
ERalpha ChIP-seq MCF7 Tamoxifen resistant cells |
| Sample type |
SRA |
| |
|
| Source name |
MCF7 breast cancer cells, Tamoxifen resistant
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7T
chip antibody: ERα antibody (HC-20) (Santa Cruz, catalog# sc-543)
passage: <10
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.
NEB ultra kit
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Basecalls performed using CASAVA version 1.4
Alignment was performed with Bowtie v1.1.2
Peak calling was perfromed using MACS 2.0
Genome_build: Hg19
Supplementary_files_format_and_content: bed file reports peaks
|
| |
|
| Submission date |
Feb 16, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Luca Magnani |
| E-mail(s) |
magnanirimini78@gmail.com
|
| Organization name |
Imperial College London
|
| Department |
Surgery and Cancer
|
| Lab |
Magnani's Lab
|
| Street address |
DuCane road
|
| City |
London |
| ZIP/Postal code |
W120NN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE60517 |
Drug specific epigenetic reprogramming leads to increased cellular invasion in ERα positive breast cancer via de novo cholesterol biosynthesis. |
|
| Relations |
| SRA |
SRX1585243 |
| BioSample |
SAMN04504413 |
