|
Status |
Public on Sep 26, 2016 |
Title |
697 RNA-Seq |
Sample type |
SRA |
|
|
Source name |
697 cell line
|
Organism |
Homo sapiens |
Characteristics |
cell line: 697 cell type: B-ALL
|
Treatment protocol |
Cells were seeded at 1*10^6 cells/ml.
|
Growth protocol |
Cells were grown in RPMI supplemented with 10% fetal bovine serum, 1% glutamin and 1% penicilin/streptomycin
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was harvested with the miRNeasy mini kit (Qiagen) with Dnase treatment on-column. totalRNA, ribodepletion, stranded RNA-seq
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Data processing |
samples were aligned to hg19 using STAR2.4.2a and ensembl GRCh37v75 as guide Genome_build: hg19 Supplementary_files_format_and_content: STAR count output
|
|
|
Submission date |
Apr 04, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Farzaneh Ghazavi |
E-mail(s) |
farzaneh.ghazavi@ugent.be
|
Organization name |
Gent university hospital
|
Department |
Center of medical genetics
|
Street address |
De Pintelaan 185
|
City |
Ghent |
ZIP/Postal code |
9000 |
Country |
Belgium |
|
|
Platform ID |
GPL11154 |
Series (2) |
GSE79871 |
RNA-sequencing of two the B-ALL cell lines, REH and 697 |
GSE79873 |
Unique long non-coding RNA expression signature in ETV6/RUNX1-driven B-cell precursor acute lymphoblastic leukemia |
|
Relations |
BioSample |
SAMN04605048 |
SRA |
SRX1677206 |