|
| Status |
Public on May 29, 2008 |
| Title |
SG_1P{hsGAL4}89(S)_14APF_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
GAL4-expressing salivary glands, heat shocked, dissected 14 hours APF
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Salivary glands of animals carrying one copy of the heat-inducible transgene P{GAL4-Hsp70.PB}89-2-1, heat shocked, dissected at 14 hours after puparium formation.
|
| Treatment protocol |
Animals were subjected to a 30-min heat shock treatment at 37 °C at 9 hours after puparium formation. Salivary glands were dissected for RNA extraction at 14 hours after puparium formation.
|
| Growth protocol |
Animals were kept at 25 °C, except during heat shock period.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Salivary glands were dissected on ice and directly transferred to TRIzol Reagent (Invitrogen, Carlsbad, California). To support lysis of the tissue, the mixture was subjected to vigorous vortexing. Total RNA was isolated according to the instructions of the manufacturer. As a last step of the extraction procedure, the RNA was purified using an RNeasy Minikit (Quiagen, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cRNA was produced using the One-Cycle Eukaryotic Target Labeling Assay recommended by Affymetrix. Labeling was carried out exactly as specified in the instructions manual, section 2 (http://www.affymetrix.com/support/technical/manual/expression_manual.affx).
|
| |
|
| Hybridization protocol |
Hybridization was performed following the instructions provided in the Affymetrix Manual (Section 2), using a Fluidics Station 450 and the Midi_euk2 v3 protocol.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Genechip 3000 Scanner 7G and Genechip Operation Software (GCOS) v. 1.4.
|
| Description |
Salivary glands from animals carrying one copy of the heat-inducible transgene P{GAL4-Hsp70.PB}89-2-1, heat shocked at 9 hours after puparium formation, and dissected at 14 hours after puparium formation.
|
| Data processing |
The data were processed and normalized using dChip 2006. The median intensity of the baseline assay used for normalization was 150.
|
| |
|
| Submission date |
Jul 27, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Michael Lehmann |
| E-mail(s) |
mlehmann@uark.edu
|
| Phone |
479-575-3688
|
| Organization name |
University of Arkansas
|
| Department |
Biological Sciences
|
| Street address |
SCEN 601
|
| City |
Fayetteville |
| State/province |
AR |
| ZIP/Postal code |
72701 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (2) |
| GSE8619 |
Senseless-responsive genes in larval salivary glands of late Drosophila prepupae |
| GSE8623 |
Senseless-responsive and GAL4-responsive genes in larval salivary glands of late Drosophila prepupae |
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