|
| Status |
Public on Jul 04, 2016 |
| Title |
pES2 |
| Sample type |
SRA |
| |
|
| Source name |
pES2 Human embryonic stem cells
|
| Organism |
Homo sapiens |
| Characteristics |
differentiation status: Undifferentiated
karyotype: Parthenogenetic
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted using NucleoSpin RNA Plus kit (Marcherey-Nagel). mRNA was enriched by Poly-A selection.
Sequencing libraries were prepared using TruSeq RNA Library Prep Kit v2 (Illumina).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Reads were mapped to the human reference genome (GRCh38/hg38) using TopHat (version 2.0.8b)
Reads per kilobase of transcript per million mapped reads (FPKM) values were calculated using Cufflinks (version 2.1.1)
Genome_build: USCS/GRCh38/hg38
Supplementary_files_format_and_content: Tab-delimited text file includes FPKM values for each sample
|
| |
|
| Submission date |
May 13, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Uri Weissbein |
| E-mail(s) |
uriwsbn@gmail.com
|
| Organization name |
Hebrew University of Jerusalem
|
| Department |
Genetics
|
| Lab |
Nissim Benvenisty Lab
|
| Street address |
Haalon 4
|
| City |
Pardess hana |
| State/province |
Please Select |
| ZIP/Postal code |
37023 |
| Country |
Israel |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE81402 |
Analysis of chromosomal aberrations and recombination by allelic bias in RNA-Seq |
|
| Relations |
| BioSample |
SAMN04994876 |
| SRA |
SRX1759177 |
