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Status |
Public on Jun 28, 2021 |
Title |
Huh7.5.1_HCV_captopril_1uM_rep1 |
Sample type |
RNA |
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Source name |
Huh7.5.1, HCV, captopril
|
Organism |
Homo sapiens |
Characteristics |
cell line: Huh7.5.1 cell type: DMSO-differentiated Huh7.5.1 cells (Huh7.5.1dif) exposure with etiological agent: HCV Jc1 infection (TCID50/mL=10^6/mL) duration with etiological agent: 10 days intervention: Captopril (1 microM) duration of intervention: 3 days
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Treatment protocol |
DMSO-differentiated Huh7.5.1 cells (Huh7.5.1dif) were exposed to mock or HCV Jc1 clone, then treated with various drugs to measure modulation of Prognostic Liver Signature genes.
|
Growth protocol |
DMSO-differentiated Huh7.5.1 cells (Huh7.5.1dif) were cultured in DMEM with 1% DMSO for 10 days.
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Extracted molecule |
total RNA |
Extraction protocol |
Cells were washed with PBS and lysed with 5 µl of iScript™ RT-qPCR Sample Preparation Reagent (Bio-rad, Hercules, California).
|
Label |
biotin
|
Label protocol |
Refer to the NanoString nCounter Assay System Manual.
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|
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Hybridization protocol |
Refer to the NanoString nCounter Assay System Manual.
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Scan protocol |
Refer to the NanoString nCounter Assay System Manual.
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Description |
Capto 1-1
|
Data processing |
For each probe, transcript count data were log2 transformed and scaled by geometric mean of the normalization genes by NanoString normalizer module of GenePattern toolkit (www.broadinstitute.org/genepattern) after setting a floor value of 1.1.
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Submission date |
May 23, 2016 |
Last update date |
Jun 28, 2021 |
Contact name |
Yujin Hoshida |
E-mail(s) |
Yujin.Hoshida@UTSouthwestern.edu
|
Organization name |
University of Texas Southwestern Medical Center
|
Street address |
5323 Harry Hines Blvd
|
City |
Dallas |
State/province |
TX |
ZIP/Postal code |
75390 |
Country |
USA |
|
|
Platform ID |
GPL19886 |
Series (2) |
GSE66843 |
A cell-based model unravels drivers for hepatocarcinogenesis and targets for clinical chemoprevention |
GSE81801 |
Prognostic Liver Signature profiles of Jc1-infected Huh7.5.1dif cells treated with various drugs |
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