|
| Status |
Public on Jan 01, 2017 |
| Title |
CD36OE-2 |
| Sample type |
SRA |
| |
|
| Source name |
HepG2.2.15-CD36 overexpression cell
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HepG2.2.15
lentivirus: CD36 overexpression
|
| Treatment protocol |
CD36-overexpression-lentivirus and Vector-lentivirus were transfected into HepG2.2.15 cells.
|
| Growth protocol |
Modified Eagle Medium (MEM) containing 10% fetal calf serum and 500g of G418/ml.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Agilent) and kept at -80℃. The RNA with RIN >8.0 is right for cDNA library construction.
Ion Proton Sequencing Protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Ion Torrent Proton |
| |
|
| Description |
Processed data file: All_Counts.txt
|
| Data processing |
Base calling using Torrent Suite Software and Torrent Server.
Reads filtering under criteria removing reads with 20% of the base quality lower than 13.
Mapsplice v2.1.8 mapping to reference genome.
Reads counting using HTSeq.
Genome_build: hg19 (GRCh37)
Supplementary_files_format_and_content: All_Counts.txt: Tab-delimited text file contains raw counts for each sample.
|
| |
|
| Submission date |
Jun 21, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
健 黄 |
| E-mail(s) |
zmchj2001@163.com
|
| Phone |
15086067610
|
| Organization name |
遵义医学院附属医院
|
| Street address |
汇川区大连路149号遵义医学院附属医院
|
| City |
遵义市 |
| State/province |
贵州省 |
| ZIP/Postal code |
563003 |
| Country |
China |
| |
|
| Platform ID |
GPL17303 |
| Series (1) |
| GSE83577 |
Genome-wide Transcriptome Analysis of CD36 Overexpression in HepG2.2.15 Cells to Explore Its Regulation Role of Metabolism and HBV Life Cycle |
|
| Relations |
| BioSample |
SAMN05277756 |
| SRA |
SRX1868805 |
