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| Status |
Public on Jul 15, 2016 |
| Title |
MS417-3hr (RNA-seq) |
| Sample type |
SRA |
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| Source name |
t(8;21)-containing acute myeloid leukemia cell line
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| Organism |
Homo sapiens |
| Characteristics |
cell line: Kasumi-1 cells
treatment: 125 nM MS417, 3 hr
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| Treatment protocol |
125 nM MS417, 3 hr
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| Growth protocol |
RPMI 1640 supplemented with 10% FetalPlex and 2 mM L-Glutamine
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
TRIzol extraction
PolyA enriched RNA was used to build library using TruSeq RNA Library Preparation Kit.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
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| Data processing |
Base-calling, filtering, and demultiplexing were performed using Illumina CASAVA 1.8.2 with default settings.
Raw reads were processed to trim adaptor and low quality sequences using Trimmomatic (version 0.32) with parameters: -phred33 input.txt.gz output.txt.gz ILLUMINACLIP:adaptor.txt:2:30:7 TRAILING:15.
For PRO-seq, reverse complements of the trimmed reads were generated using “fastx_reverse_complement” (version 0.0.13) with default settings, and the reverse complement sequences were aligned to the human genome hg19 using bowtie2 (version 2.2.4) with default settings. For ChIP-exo, pre-processed reads were aligned to hg19 using bowtie2 with default settings. For RNA-seq, pre-processed reads were aligned to hg19 using TopHat (version 2.0.10) with default settings.
H3K27ac peak calling was performed using MACS2 (version 2.0.10) with parameters: MACS2 callpeak -t H3K27ac.bam -c Input.bam --nomodel -f BAM -g hs -n -B.
Genome_build: hg19
Supplementary_files_format_and_content: Genome coverage was normalized and calculated for each strand separately and reported in bedGraph format. The ChIP-exo peak file reports genome coordinates, peak length, read counts, and enrichment significance for each called H3K27ac peak. RNA-seq count files contain gene RefSeq IDs and their normalized read counts in reads per kilobase of transcript per million mapped reads (RPKM).
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| Submission date |
Jun 23, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Scott W Hiebert |
| E-mail(s) |
scott.hiebert@vanderbilt.edu
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| Phone |
615-936-3582
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| Organization name |
Vanderbilt University
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| Department |
Biochemistry
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| Lab |
Hiebert Lab
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| Street address |
2220 Pierce Ave
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| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
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| Platform ID |
GPL11154 |
| Series (1) |
| GSE83660 |
High Resolution Mapping of RNA Polymerases Identifies Mechanisms of Sensitivity and Resistance to BET Inhibitors in t(8;21) AML |
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| Relations |
| BioSample |
SAMN05290360 |
| SRA |
SRX1873245 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM2212044_RNAseq-Kasumi1-MS417-3hr-normalized-count.txt.gz |
77.0 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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