|
| Status |
Public on Apr 01, 2018 |
| Title |
KRAS_G12D |
| Sample type |
RNA |
| |
|
| Source name |
C2C12
|
| Organism |
Mus musculus |
| Characteristics |
days in differentiation media: 5
|
| Treatment protocol |
Transduced cells were incubated in DMEM + 2% horse serum for 5 days prior to RNA extraction. Media was replaced daily.
|
| Growth protocol |
C2C12 cells were transduced with retrovirus and selected with puromycin. Expression of RAS was verified by immunoblot.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Qiagen RNeasy mini kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on the Affymetrix mouse genome 430 2.0 array.
|
| Scan protocol |
GeneChips were scanned according to standard protocols at the Genomics Technology Laboaratory
|
| Description |
gene expression data from C2C12 myotubes
|
| Data processing |
The data were normalized by Affymetrix Power Tool
|
| |
|
| Submission date |
Aug 03, 2016 |
| Last update date |
Apr 01, 2018 |
| Contact name |
Javed Khan |
| E-mail(s) |
khanjav@mail.nih.gov
|
| Phone |
2407606135
|
| Organization name |
NCI, NIH
|
| Department |
Genetics Branch
|
| Lab |
Javed Khan
|
| Street address |
37 Convent Dr.
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE85168 |
Oncogenic RAS blocks myogenic differentiation |
| GSE85171 |
Epigenetic Reprogramming of mutant RAS-driven Rhabdomyosarcoma via MEK Inhibition |
|
