|
| Status |
Public on Jan 31, 2008 |
| Title |
shRNA_3D_1 |
| Sample type |
RNA |
| |
|
| Source name |
SATB1-shRNA trasnfected MDA-MB-231 cells grown on 3D culture
|
| Organism |
Homo sapiens |
| Characteristics |
SATB1-knocked down (by shRNA) human breast cancer cell line
|
| Treatment protocol |
MDA-MB-231 cells stably trasnfected with pSUPER_ SATB1-shRNA1was cloned.
|
| Growth protocol |
grown on matrigel coated culture dish (3D culture)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ?g total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ?g of cRNA were hybridized for 16 hr at 45C.
|
| Scan protocol |
GeneChips were scanned according to manufacturer's instructions (2001 Affymetrix Genechip technical Manual)
|
| Description |
Gene expression data from MDA-MB-231-SATB1_shRNA1 cultured on matrigel coated dishes (3D)
|
| Data processing |
Probe set based gene expression measurements were generated from .CEL files using the RMA algorithm from the GeneSpring software (SiLicon Genetics).
|
| |
|
| Submission date |
Sep 12, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Terumi Kohwi-shigematsu |
| E-mail(s) |
terumiks@lbl.gov
|
| Phone |
510-486-4983
|
| Fax |
510-486-4545
|
| Organization name |
LBNL
|
| Department |
Life Science
|
| Street address |
1 Cyclotron road
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720 |
| Country |
USA |
| |
|
| Platform ID |
GPL3921 |
| Series (1) |
| GSE5417 |
Expression data of control-shRNA and SATB1-shRNA expressing MDA-MB-231 cells from 2D and 3D culture system |
|
