|
| Status |
Public on Aug 29, 2018 |
| Title |
HUVEC Notch1 siRNA_3 |
| Sample type |
RNA |
| |
|
| Source name |
Human umbilical vein endothelial cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Human umbilical vein endothelial cells
sirna: Notch1
stimulation: medium
|
| Treatment protocol |
Cells were seeded 37.500 cells/cm2, cultured for 24 hours and transfected with siRNA (scrambled or targeting Notch1). 48 hours after transfection, cells were stimulated with recombinant human interleukin-1b (5ng/ml, control cells were given fresh medium) for 4 hours before harvesting in Qiagen RLT lysis buffer.
|
| Growth protocol |
Pooled HUVEC from 10 donors were cultured in MCDB 131 with 7.5% FBS, 10 ng/ml EGF, 1 ng/ml bFGF, 1μg/ml hydrocortisone, 50 μg/ml gentamicin, 250 ng/ml fungizone and 1% L-glutamine. Cells were maintained at 37°C in human 95% air/ 5% CO2 atmosphere.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Qiagen RNEasy Mini kit, according to manufacturers instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina® TotalPrep™-96 RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
Raw data (.idat files) were read and processed by R/Bioconductor/beadarray
|
| |
|
| Submission date |
Aug 24, 2016 |
| Last update date |
Aug 29, 2018 |
| Contact name |
Guttorm Haraldsen |
| E-mail(s) |
guttorm.haraldsen@rr-research.no
|
| Organization name |
Oslo University Hospital
|
| Department |
Department for Pathology
|
| Lab |
LIIPAT
|
| Street address |
Sognsvannsveien 20
|
| City |
OSLO |
| State/province |
OSLO |
| ZIP/Postal code |
0027 |
| Country |
Norway |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE85986 |
Effect of Notch1 on inflammatory activation of human umbilical vein endothelial cells |
| GSE85987 |
Inhibition of endothelial Notch signaling attenuates inflammation |
|
