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Sample GSM2290203 Query DataSets for GSM2290203
Status Public on Aug 29, 2018
Title Endothelial cells isolated from DNFB-treated ear, NICD1-EC-OE mouse, replicate 1
Sample type RNA
 
Source name CD31+CD45-Ter119- endothelial cells, FACS-sorted from collagenase I-digested ears.
Organism Mus musculus
Characteristics tissue: ear
cell population: CD31+CD45-Ter119- endothelial cells
genotype/variation: Pdgfb-CreERT2; R26-NICD
Treatment protocol Mice were treated with 5 daily tamoxifen injections before the start of experiment. DNFB was applied dermally on two consecutive days (day 0 and 1) to promote sensitisation. Inflammation was elicited by a single application of DNFB to the ear pinna on day 5. Ears were harvested after 24 hours and digested in collagenase I. FACS was performed to isolate CD31+CD45-Ter119- endothelial cells. Cells were sorted directly into Qiagen RLT Plus lysis buffer.
Growth protocol NICDiEC-OE mice with endothelial expression of constitutively active Notch1 were generated by combining RosaNICD/NICD with CDH5-iCre transgenics.
Extracted molecule total RNA
Extraction protocol RNA was extracted using the Qiagen RNEasy Plus Micro kit and amplified using the Affymetrix WT Pico Amplification kit, according to manufacturers description.
Label biotin
Label protocol Biotinylated cRNA were prepared from 1ng total RNA and amplified according to manufacturers instructions, using the Affymetric GeneChip WT Pico kit.
 
Hybridization protocol Following fragmentation and terminal labeling, 5,5 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Gene Chip Mouse Gene 2.1 ST array. GeneChips were washed and stained in the Affymetrix GeneCips fluidics station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneTitan Multichannel instrument.
Description Gene expression data of endothelial cells from DNFB treated ears from mice with tamoxifen-induced endothelial overexpression of constitutively active Notch1
Data processing RMA-normalised data were obtained using the Affymetrix Expression Console Software.
 
Submission date Aug 24, 2016
Last update date Aug 29, 2018
Contact name Guttorm Haraldsen
E-mail(s) guttorm.haraldsen@rr-research.no
Organization name Oslo University Hospital
Department Department for Pathology
Lab LIIPAT
Street address Sognsvannsveien 20
City OSLO
State/province OSLO
ZIP/Postal code 0027
Country Norway
 
Platform ID GPL17400
Series (2)
GSE85987 Inhibition of endothelial Notch signaling attenuates inflammation
GSE85992 Gene expression data from endothelial cells isolated from DNFB-treated ears of mice with inducible endothelial-specific overexpression of constitutively active Notch1 intracellular domain

Data table header descriptions
ID_REF
VALUE RMA-normalised signal intensity

Data table
ID_REF VALUE
17210850 2.646492
17210852 2.323917
17210855 7.401496
17210869 7.188241
17210883 2.443151
17210887 7.0199
17210904 2.513789
17210912 6.513776
17210947 2.631678
17210953 2.947986
17210984 6.515553
17210994 3.735275
17210996 4.00266
17210998 2.40063
17211000 7.795624
17211004 4.806777
17211023 3.415872
17211033 2.778316
17211043 6.799155
17211066 2.643024

Total number of rows: 41345

Table truncated, full table size 722 Kbytes.




Supplementary file Size Download File type/resource
GSM2290203_A3291-25.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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