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| Status |
Public on Jan 29, 2008 |
| Title |
Glioblastoma-Gbm 77 |
| Sample type |
RNA |
| |
|
| Source name |
glioma
|
| Organism |
Homo sapiens |
| Characteristics |
male 55y, ampl EGFR, expr EGFRvIII
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA, purification on RNeasy mini columns (Qiagen)
|
| Label |
biotin
|
| Label protocol |
labelling was performed according to the Affymetrix (Santa Clara, CA) GeneChip Whole Transcript (WT) Sense Target Labelling Assay manual
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| |
|
| Hybridization protocol |
microarrays were hybridized overnight with 5 g biotin labelled ss-cDNA, and washed in fluidics station wash protocol: MES_EukGE-WS2v5_450
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| Scan protocol |
Genechip scanner 3000 7G
|
| Description |
examination of differentially regulated splice variants and novel exons in glial brain tumors
|
| Data processing |
GCOS, data extraction in ExACT. Plier data is provided in sample table.
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| |
|
| Submission date |
Oct 19, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Pim French |
| E-mail(s) |
p.french@erasmusmc.nl
|
| Phone |
+31 10 70443 33
|
| Organization name |
Erasmus MC
|
| Department |
Neurology
|
| Lab |
Neurooncology
|
| Street address |
Dr Molewaterplein 50
|
| City |
Rotterdam |
| ZIP/Postal code |
2040CA |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL5188 |
| Series (1) |
| GSE9385 |
Identification of differentially regulated splice variants and novel exons in glial brain tumors using exon arrays |
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| Data table header descriptions |
| ID_REF |
|
| VALUE |
plier data |
| Data table |
| ID_REF |
VALUE |
| 2590411 |
33 |
| 2609210 |
40 |
| 2616957 |
68 |
| 2391254 |
6 |
| 3499280 |
0 |
| 2807653 |
0 |
| 2532280 |
51 |
| 3842613 |
11 |
| 3871433 |
49 |
| 3087946 |
24 |
| 3234450 |
0 |
| 3452435 |
46 |
| 2580386 |
13 |
| 3949778 |
120 |
| 2689727 |
6 |
| 2846401 |
84 |
| 2385421 |
21 |
| 2992747 |
4 |
| 3432063 |
55 |
| 2827457 |
22 |
Total number of rows: 1411354
Table truncated, full table size 14923 Kbytes.
| Supplementary file |
Size |
Download |
File type/resource |
| GSM238909.CEL.gz |
20.7 Mb |
(ftp)(http) |
CEL |
| Processed data provided as supplementary file |
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