|
| Status |
Public on Jan 17, 2008 |
| Title |
Luc_72_hr_B |
| Sample type |
RNA |
| |
|
| Source name |
CD34+ cells infected with lentivirus expressing luciferase shRNA, harvested 72 hours after infection
|
| Organism |
Homo sapiens |
| Characteristics |
CD34+ cells infected with lentivirus expressing luciferase shRNA, harvested 72 hours after infection
|
| Treatment protocol |
shRNAs were introduced into normal CD34+ human hematopoietic progenitor cells,
|
| Growth protocol |
CD34+ cells were induced to differentiate for 10 days along the erythroid and megakaryocytic lineages.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified from mononuclear cells using Trizol (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Linear amplification of 20 ng of total RNA was performed using the Ovation Biotin RNA Amplification and Labeling System (Nugen, Inc.).
|
| |
|
| Hybridization protocol |
cDNA was hybridized to HG_U133AAofAv2 microarrays following standard Affymetrix procedure.
|
| Scan protocol |
Affymetrix
|
| Description |
CD34+ cells infected with lentivirus expressing luciferase shRNA, harvested 72 hours after infection
|
| Data processing |
Raw expression values were normalized using Robust Multiarray Averaging (RMA)
|
| |
|
| Submission date |
Oct 31, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Pablo Tamayo |
| E-mail(s) |
ptamayo@ucsd.edu
|
| Phone |
508-259-0630
|
| Organization name |
UCSD
|
| Department |
Moores Cancer Center
|
| Street address |
3855 Health Sciences Drive MC 0658
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platform ID |
GPL3921 |
| Series (1) |
| GSE9487 |
Genome-wide expression profiling of cells infected with control or RPS14 shRNAs |
|
