|
Status |
Public on Jan 17, 2008 |
Title |
RPS14_G4_72_hr_B |
Sample type |
RNA |
|
|
Source name |
CD34+ cells infected with lentivirus expressing RPS14_G4 shRNA, harvested 72 hours after infection
|
Organism |
Homo sapiens |
Characteristics |
CD34+ cells infected with lentivirus expressing RPS14_G4 shRNA, harvested 72 hours after infection
|
Treatment protocol |
shRNAs were introduced into normal CD34+ human hematopoietic progenitor cells,
|
Growth protocol |
CD34+ cells were induced to differentiate for 10 days along the erythroid and megakaryocytic lineages.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was purified from mononuclear cells using Trizol (Invitrogen).
|
Label |
biotin
|
Label protocol |
Linear amplification of 20 ng of total RNA was performed using the Ovation Biotin RNA Amplification and Labeling System (Nugen, Inc.).
|
|
|
Hybridization protocol |
cDNA was hybridized to HG_U133AAofAv2 microarrays following standard Affymetrix procedure.
|
Scan protocol |
Affymetrix
|
Description |
CD34+ cells infected with lentivirus expressing RPS14_G4 shRNA, harvested 72 hours after infection
|
Data processing |
Raw expression values were normalized using Robust Multiarray Averaging (RMA)
|
|
|
Submission date |
Oct 31, 2007 |
Last update date |
Aug 14, 2011 |
Contact name |
Pablo Tamayo |
E-mail(s) |
ptamayo@ucsd.edu
|
Phone |
508-259-0630
|
Organization name |
UCSD
|
Department |
Moores Cancer Center
|
Street address |
3855 Health Sciences Drive MC 0658
|
City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92093 |
Country |
USA |
|
|
Platform ID |
GPL3921 |
Series (1) |
GSE9487 |
Genome-wide expression profiling of cells infected with control or RPS14 shRNAs |
|