|
| Status |
Public on Oct 31, 2017 |
| Title |
FLS_RA_basal_2 |
| Sample type |
RNA |
| |
|
| Source name |
RA-FLS_basal
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: Rheumatoid Arthritis patient
cell type: Fibroblast like synoviocyte (FLS)
stimulated with: none
|
| Treatment protocol |
Cultured FLS were stimualted with 5%FBS DMEM which containted 10ng/ml of IL-1beta or not for 12 hours.
|
| Growth protocol |
FLS were isolated from synovial tissues of patients with rheumatoid arthritis or osteoarthritis and cultured in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 6.0g/L of HEPES, 0.4g/L of L-glutamine, 3.7g/L of Sodium bicarbonate, 1X of antibiotics, 1X of MEM Non-Essential Amino Acids, and 1X of 2-Mercaptoethanol. Experiments were performed between passage 3 and 9.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
We prepared total RNA independently from two (for miRNA) or three (for mRNA) replicate samples using the RNeasy mini kit (Qiagen, Hilden, Germany) and assessed RNA integrity using Bioanalyzer 2100 (Agilent Technologies, CA). The RNA integrity number (PRN) was close to 10 for all samples.
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared, followed by RNAeasy Mini Kit (QIAGEN). cRNA was quantified and normalized by using NanoDrop
|
| |
|
| Hybridization protocol |
RNA was then amplified and hybridized to Agilent-019118 Human miRNA Microarray 2.0 G4470B according to the manufacturers’ standard protocols.
|
| Scan protocol |
cRNA on Agilent SurePrint G3 Human Gene Expression 8x60K v2 microarray chip was scanned by SureScan Microarray Scanner
|
| Description |
RA-FLS 2-57
|
| Data processing |
Quantile-normalization was performed for the log2-value of gMedianSignal
|
| |
|
| Submission date |
Dec 08, 2016 |
| Last update date |
Oct 31, 2017 |
| Contact name |
Bong-Ki Hong |
| E-mail(s) |
bangk82@hotmail.com
|
| Phone |
+82-2-2258-7831
|
| Organization name |
Catholic University of Korea
|
| Department |
School of Medicine
|
| Street address |
222 Banpo-daero, Seocho-gu
|
| City |
Seoul |
| ZIP/Postal code |
06591 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL14943 |
| Series (1) |
| GSE91026 |
Global miRNA expression profiles of fibroblast-like synoviocytes in patients with rheumatoid arthritis and osteoarthritis |
|
