|
| Status |
Public on Jan 15, 2008 |
| Title |
HMLE_shEcad+shBcat_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
HMLE cells (Immortalized human mammary epithelial cells, SV40 - largeT/smallT, hTERT-immortalized), expressing shRNA against E-cadherin and shRNA against Beta-catenin
|
| Organism |
Homo sapiens |
| Characteristics |
Gender: female, Age: Premenopausal, Tissue: Normal Breast
|
| Treatment protocol |
Immortalized by SV40 - largeT/smallT in MEGM medium
|
| Growth protocol |
in vitro cultured in MEGM medium on regular culture plates
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from culture plates for each cell type using an RNeasy Mini kit coupled with an RNase-free DNase set (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol; hybridization was performed with high-throughput HT_HG-U133A arrays in 96-well format
|
| Scan protocol |
Scanned using an HT Scanner
|
| Description |
Gene expression changes induced by knockdown of E-cadherin, expression of dominant-negative E-cadherin and double knockdown of E-cadherin and Beta-catenin in immortalized human mammary epithelial cells
|
| Data processing |
Raw data files (.CEL) were converted into probe-set values using RMA normalization.
|
| |
|
| Submission date |
Nov 26, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Tamer T Onder |
| Organization name |
Children's Hospital Boston
|
| Street address |
One Blackfan Circle
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL3921 |
| Series (1) |
| GSE9691 |
Analysis of the effects of loss of E-cadherin and cell adhesion on human mammary epithelial cells |
|
