|
Status |
Public on Jan 15, 2008 |
Title |
HMLE_DNEcad_rep3 |
Sample type |
RNA |
|
|
Source name |
HMLE cells (Immortalized human mammary epithelial cells, SV40 - largeT/smallT, hTERT-immortalized) expressing dominant-negative E-cadherin
|
Organism |
Homo sapiens |
Characteristics |
Gender: female, Age: Premenopausal, Tissue: Normal Breast
|
Treatment protocol |
Immortalized by SV40 - largeT/smallT in MEGM medium
|
Growth protocol |
in vitro cultured in MEGM medium on regular culture plates
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from culture plates for each cell type using an RNeasy Mini kit coupled with an RNase-free DNase set (Qiagen).
|
Label |
Biotin
|
Label protocol |
Standard Affymetrix protocol
|
|
|
Hybridization protocol |
Standard Affymetrix protocol; hybridization was performed with high-throughput HT_HG-U133A arrays in 96-well format
|
Scan protocol |
Scanned using an HT Scanner
|
Description |
Gene expression changes induced by knockdown of E-cadherin, expression of dominant-negative E-cadherin and double knockdown of E-cadherin and Beta-catenin in immortalized human mammary epithelial cells
|
Data processing |
Raw data files (.CEL) were converted into probe-set values using RMA normalization.
|
|
|
Submission date |
Nov 26, 2007 |
Last update date |
Aug 14, 2011 |
Contact name |
Tamer T Onder |
Organization name |
Children's Hospital Boston
|
Street address |
One Blackfan Circle
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL3921 |
Series (1) |
GSE9691 |
Analysis of the effects of loss of E-cadherin and cell adhesion on human mammary epithelial cells |
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