|
| Status |
Public on Jan 11, 2018 |
| Title |
JG-98 2 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7_JG-98
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7
treated with: 2 uM JG-98 for 24hrs
|
| Treatment protocol |
Cells were treated with 2 micromoles of JG-98 for 24 hours.
|
| Growth protocol |
MCF7 cells were grown on plates in DMEM + 10%FBS till 40% density.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mRNA was extracted using the Qiagen kit.
|
| Label |
biotin
|
| Label protocol |
Biotin labeling was performed using the Ambion WT Expression Kit (Life Technologies, Grand Island, NY) according to the manufacturer's protocol, followed by the GeneChip WT Terminal Labeling and Controls Kit (Affymetrix, Santa Clara, CA).
|
| |
|
| Hybridization protocol |
The labeled, fragmented DNA was hybridized to the Affymetrix Human Gene 2.0 ST Array for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450.
|
| Scan protocol |
After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus.
|
| Description |
JG 2
|
| Data processing |
Raw Affymetrix CEL files were normalized to produce Entrez Gene-identifier-specific expression values using the implementation of the Robust Multiarray Average (RMA) in the affy Bioconductor package (version 1.36.1), using R version 2.15.1 and the Brainarray hugene20sthsentrezg R package (version 17.0.0).
|
| |
|
| Submission date |
Jan 12, 2017 |
| Last update date |
Jun 25, 2019 |
| Contact name |
Boston University Microarray and Sequencing Resource |
| E-mail(s) |
msrdata@bu.edu
|
| Organization name |
Boston University
|
| Department |
Microarray and Sequencing Resource
|
| Street address |
72 East Concord Street, E631
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
| |
|
| Platform ID |
GPL17930 |
| Series (1) |
| GSE93542 |
Effect of the Hsp70 inhibitor JG-98 on gene expression in MCF7 cells |
|
