|
| Status |
Public on Feb 01, 2008 |
| Title |
Brain_ProtocolG_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Ambion Human Brain Reference RNA (Catalog #6050)
|
| Organism |
Homo sapiens |
| Characteristics |
Brain_ProtocolG_rep2
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA extracted
|
| Label |
biotin
|
| Label protocol |
100ng of total RNA were amplified and labeled using the Whole Transcript (WT) Sense Target Labeling Protocol
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip® Human Gene 1.0 ST arrays were hybridized to 11 mg of labeled, amplified cDNA, washed, stained and scanned according to the protocol described in WT Sense Target Labeling Assay Manual (Version 4; FS450_0007)
|
| Scan protocol |
Scanning was done on an Affymetrix GeneChip Scanner 7G
|
| Description |
Samples labeling and hybridization day 1, replicate 2
|
| Data processing |
Normalized expression signals were calculated from Affymetrix CEL files using RMA (Irizarry et al., 2003).
|
| |
|
| Submission date |
Dec 07, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Sylvain Pradervand |
| E-mail(s) |
Sylvain.Pradervand@unil.ch
|
| Phone |
+41 21 692 39 08
|
| Organization name |
UNI Lausanne
|
| Department |
CIG
|
| Lab |
DNA Array Facility
|
| Street address |
Genopode
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE9819 |
Comparisons of Affymetrix Whole-Transcript Human Gene 1.0 ST array with standard 3' expression arrays |
|
