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| Status |
Public on Mar 13, 2017 |
| Title |
2794_S1 |
| Sample type |
SRA |
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| Source name |
cumulus pieces surrounding oocytes that not cleave after fertilization
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| Organism |
Bos taurus |
| Characteristics |
tissue: cumulus cells
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA of cumulus pieces was isoloated using the miCURY RNA isolation kit (Exiqon), according to the manufacturer’s protocol.
For sequencing library preparation the NEBNEXT kit (New England Biolabs, Ipswich, MA, USA) was used.
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| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina NextSeq 500 |
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| Data processing |
library generation using 3' and 5' RACE like protocols
adapter trimming and mapping of high quality data to the reference genome (bos taurus)
Genome_build: Bos_taurus_UMD_3.1.1
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| Submission date |
Feb 10, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Karen Uhde |
| E-mail(s) |
K.Uhde@uu.nl
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| Organization name |
Utrecht University
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| Street address |
Yalelaan 104
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| City |
Utrecht |
| ZIP/Postal code |
3584CM |
| Country |
Netherlands |
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| Platform ID |
GPL23055 |
| Series (1) |
| GSE94771 |
microRNA expression in bovine cumulus cells and its relation to oocyte developmental competence |
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| Relations |
| BioSample |
SAMN06319395 |
| SRA |
SRX2549935 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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